In vitro antibacterial activity of dihydroanthracene disulfonic acid derivative against enterobacter cloacae

Abstract

This paper is a report of the in vitro antibacterial activity of the dihydroanthracene disulfonic acid derivative preparation (patent DE102004003030A1) against a case diagnosed with Necrotizing Fasciitis that resulted resistant to many antibiotics. Necrotizing Fasciitis is known for his high lethality, but our in vitro study demonstrated the antibacterial effectiveness of dihydroanthracene disulfonic acid derivative. We decided to use the method of dilution in broth (Macrodilution) in the Microbiological Laboratory of the UHC “Mother Theresa” Tirana. The method consists in progressively decreasing the amount of the antimicrobial preparation in the test tubes with Mueller Hinton. In our case, we collected 12 test tubes. In the first test tube we poured a 20% solution of dihydroanthracene disulfonic acid derivative, which activity we wanted to test and in all the other test tubes we progressively decreased the amount of our preparation and added Mueller Hinton. After the dilution we incubated the 12 test tubes for 24 hours and we cultivated the above in plates with MacConkey broth for 24 hours. In vitro antibacterial effect of dihydroanthracene disulfonic acid derivative is equivalent with the in vitro effect of Imipenem (MIC<=0.25), Meropenem (MIC<=0.25), Amikacin (MIC 8), Colistin (MIC<=0.5) in the isolated strain of Enterobacter Cloacae. Further studies are needed to evaluate the full pharmacological activity of dihydroanthacene disulfonic acid derivative.